Fluorescein labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.Rhodamine labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.
This conjugate is supplied essentially free of unconjugated fluorochromes. Concanavalin A (Con A) Product # Description. Staining ofyeast cells with fluorescein-concanavalin A conjugate. The Effects of Concanavalin A on Human Oral Epithelial Cell Staining Adhesion Under Various Conditions Kyle Swovick and Andrew Gale 1/14/14 Abstract: Concanavlin A (ConA), a lectin found in jack-beans can bind to multiple sugars, as can cell receptors found on cheek epithelial cells and erythrocytes. All Rights Reserved.For the best experience on our site, be sure to turn on Javascript in your browser.VECTASTAIN ABC (Avidin-Biotin Complex Kits),VECTASTAIN ABC Kits for Western Blot Detection,Anti-Avidin / Streptavidin Amplification Antibodies,Fluorophore Conjugated Avidin/Streptavidin,Enzyme Polymer for Western Blot Detection,Mounting Media for Brightfield Microscopy (IHC),Skip to the end of the images
Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.Fluorescein labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. Yeast strains were harvested by centrifuga-tion, washedtwice with distilled water, and resuspended in distilled water to a final optical absorbancy of0.1 at 660 nm(50 Klett units, Klett-Summerson Photoelectric Colorimeter, no. Concanavalin A (Con A) is a widely used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. Concanavalin A (Con A) is one of the most widely used lectins in cell biology. 0.1 mM CaCl. Our tetramethylrhodamine conjugate of Con A exhibits the bright, orange-red fluorescence of tetramethylrhodamine (absorption/emission maxima ~555/580 nm). Staining with Concanavalin agglutinin (Con A) reveals a far greater number of macrophage-histiocytes (M-H) in paraffin sections than any other staining method. (See succinylated Con A). With Con A staining, the shapes of stromal M-H are clearly visualized, thus enabling a study of their morphologic variations. This conjugate is supplied essentially free of unconjugated fluorochromes.At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa.
ConA is a plant mitogen, and is known for its ability to stimulate mouse T-cell subsets giving rise to … 0.08% sodium azide, 0.1 mM CaCl. All Rights Reserved.For the best experience on our site, be sure to turn on Javascript in your browser.VECTASTAIN ABC (Avidin-Biotin Complex Kits),VECTASTAIN ABC Kits for Western Blot Detection,Anti-Avidin / Streptavidin Amplification Antibodies,Fluorophore Conjugated Avidin/Streptavidin,Enzyme Polymer for Western Blot Detection,Mounting Media for Brightfield Microscopy (IHC),Skip to the end of the images
This conjugate is supplied essentially free of unconjugated fluorochromes. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.Copyright 2020 Vector Laboratories.
gallery,Skip to the beginning of the It binds specifically to certain structures found in various sugars, glycoproteins, and glycolipids, mainly internal and nonreducing terminal α-D-mannosyl and α-D-glucosyl groups.
Add to Cart. images gallery.Sign up to receive technical advice and exclusive deals directly to your inbox.The recommended concentration range for use is 5-20 µg/ml.10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide,
Thecells in 0.5-ml por- By … It is a member of the legume lectin family.
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