This mutant accumulates high levels of oleic acid and displays remarkable alterations in its seed lipid composition.
In Crop physiology, some case histories.SHIBLES, R. M., I. C. ANDERSON, and A. H. GIBSON. The analysis of GO function enrichment for imprinted genes revealed that a majority of MEGs were involved in metabolic process, suggesting these genes are likely associated with specific endospermogenesis processes. In silico promoter analysis revealed several cis acting elements associated with drought responses indicating an epigenetic mechanism of regulation for the drought response by means of a methylation/demethylation switch. Absence of ST in mango stone weevil (MSW) infested fruits provided direct evidence of the role of seed. The endosperm is parietal and free nuclear during the first few days after fertilization. The results suggest that SUS interacts with RGP in diverse sink tissues to channel UDP-glucose derived from imported sucrose into hemicellulose and/or glycoprotein/glycolipid biosynthesis.
Comparative proteomic and transcriptomic analyses were performed between large-seed ZB107 and small-seed ZB306, using isobaric tags for relative and absolute quantification (iTRAQ) and RNA-seq technologies, respectively. Curiously, the expression of the imprinted genes was not tightly
Intriguingly, protein localization predictions suggested multiple targeting to nucleus, mitochondria and chloroplasts for some methyltransferases. Polypeptides cross-reacting with antibodies to castor BC were detected in several dicotyledons and in the monocotyledons Hemerocallis fulva L. (day lily), Iris L., and Allium cepa L. (onion), but not in the Gramineae species Hordeum vulgare L. (barley) and Panicum virgatum L. (switchgrass). partitioned into the soluble, cytosolic fraction. Castor has a slow and cold-sensitive germination that negatively impact crop establishment.
Consequent quantitative PCR analysis revealed that although C5-MTase genes were differentially expressed under drought stress, all the demethylase genes analyzed in this study (RcDME, RcDML-3 and RcROS1) were significantly upregulated suggesting fine-tuning of DNA methylation and demethylation events.
Remobilization of stored compounds In this chapter, we dissect the potential regulatory mechanism of DNA methylation in castor bean seeds, emphasizing the processes of endosperm hypomethylation and CHH hypermethylation. Metabolic pathway network analysis showed that major and minor metabolic pathways regulate one another to ensure regular seed development and nutritive reserve accumulation. Additionally, we have used an off-line Hydrophilic Interaction Chromatography (HILIC) as a step of peptide fractionation preceding the Reverse Phase nanoLC coupled with a LTQ Orbitrap.
A comparison of 2S albumin genomic DNA and cDNA clones Transl. Castor bean (Ricinus communis), unlike Arabidopsis (Arabidopsis thaliana), provides an excellent model for studying seed biology in dicotyledons due to its endosperm that is relatively large and persistent throughout the seed development. Several of the genes identified in this tissue-specific whole transcriptome study have been used in transgenic plant research aimed at increasing the level of ricinoleic acid in TAG. Oil formation began 21 days after blossoming and two-thirds of the oil was synthesized in the next 20 days; the remaining one-third was formed in the last 20-day period. In castor bean, qualitative descriptions of seed filling divide it into four phases (formation of capsules, milky, waxy and maturity; Moshkin, 1986), and there are also more detailed descriptions of seed morphological development in ten stages,Ultrastructural aspects of storage of protein and phytin in the endosperm of ripening castor seeds.SOBOLEV, A. M., and N. P. BUZULUKOVA. In castor, both forms are expressed in developing and germinating endosperm and in the root but neither is expressed in the leaf. We have analysed neutral lipid fractions from other castor tissues using TLC, GLC and mass spectrometry. Meanwhile, deforestation is spiking along with COVID-19.Along with bragging rights, the nations that achieve exascale computing early will have a leg up in the scientific revolutions of the future.Image credit: Flickr/Oak Ridge National Laboratory, licensed under CC BY 2.0.NAS member Dalton Conley explains how the Vietnam War draft lotteries are a natural experiment for studying how military service affects life outcomes.A study examines the demographic and evolutionary history of extinct and living lions.Researchers examine how Atacama Desert microbes have adapted to parched environments and use rocks as a water source.Proceedings of the National Academy of Sciences,Earth, Atmospheric, and Planetary Sciences,Opinion: We have been in lockdown, but deforestation has not,Core Concept: Nascent exascale supercomputers offer promise, present challenges,Military conscription and public sector employment,How desert microbes extract water from rocks. The amino acid identities between C5-MTases and DNA demethylases from castor plant and their protein homologues from Jatropha curcas and Manihot esculenta, were 65 and 85%, respectively. The results indicate that castor capsule walls are photosynthetically active (about 15–30 % of the leaves) and contribute significantly to carbon fixation and seed yield accounting for 10 % photoassimilates towards seed weight.Monospecific antiserum was raised in rabbits to phosphoglyceromutase isolated from the developing endosperm of Ricinus communis. Accompanying seed maturation and starch accumulation, a combined regulation of ADP-Glc PPase by metabolites and phosphorylation may provide an enzyme with stable levels of activity.
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